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Abbkine Inc fluorescent secondary antibodies
Fluorescent Secondary Antibodies, supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+secondary+antibody/anti+fluorescent+secondary/pm42296822-88-43-46
Average 86 stars, based on 1 article reviews
fluorescent secondary antibodies - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

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Article Title: Cinobufagin inhibits M2-like tumor-associated macrophage polarization to attenuate the invasion and migration of lung cancer cells
Article Snippet: The next day, the corresponding fluorescent secondary antibody (1:200; cat. no. A23210, Abbkine Scientific Co., Ltd.) was added to the wells and incubated at room temperature for 1 h. DAPI (10 μ g/ml) staining was performed for 5 min at room temperature in the dark, followed by imaging using a laser confocal microscope.

Article Title: Cinobufagin inhibits M2‑like tumor‑associated macrophage polarization to attenuate the invasion and migration of lung cancer cells.
Article Snippet: Macrophages have crucial roles in immune responses and tumor progression, exhibiting diverse pheno‐ types based on environmental cues.. In the present study, the impact of cinobufagin (CB) on macrophage polarization and the consequences on tumor‐associated behaviors were investigated.. Morphological transformations of THP‐1 cells into M0, M1 and M2 macrophages were observed, including distinct changes in the size, shape and adherence properties of these cells.

Immunofluorescence:

Article Title: Histone demethylase JMJD1A protects mice from enteric bacterial infection by upregulating CCL8 expression to recruit macrophages and CD4 + T cells
Article Snippet: For immunofluorescence staining (IF), the antibodies Rabbit anti-Mouse-CCL8 antibody (Cat#AF790, R&D Systems) was incubated for overnight at 4 °C. .. The Fluorescent Secondary Antibody (Cat#A23330, Abbkine) for immunofluorescence was incubated for 30 min. .. Slides were mounted with the Antifade Mounting Medium with DAPI (Cat# H-1200, Vector Laboratories, USA).

Article Title: Histone demethylase JMJD1A protects mice from enteric bacterial infection by upregulating CCL8 expression to recruit macrophages and CD4+ T cells.
Article Snippet: For immunofluorescence staining (IF), the antibodies Rabbit anti-Mouse-CCL8 antibody (Cat#AF790, R&D Systems) was incubated for overnight at 4 °C. .. The Fluorescent Secondary Antibody (Cat#A23330, Abbkine) for immunofluorescence was incubated for 30 min. .. Slides were mounted with the Antifade Mounting Medium with DAPI (Cat# H-1200, Vector Laboratories, USA).

Incubation:

Article Title: Histone demethylase JMJD1A protects mice from enteric bacterial infection by upregulating CCL8 expression to recruit macrophages and CD4 + T cells
Article Snippet: For immunofluorescence staining (IF), the antibodies Rabbit anti-Mouse-CCL8 antibody (Cat#AF790, R&D Systems) was incubated for overnight at 4 °C. .. The Fluorescent Secondary Antibody (Cat#A23330, Abbkine) for immunofluorescence was incubated for 30 min. .. Slides were mounted with the Antifade Mounting Medium with DAPI (Cat# H-1200, Vector Laboratories, USA).

Article Title: TRPM4 modulates endothelial inflammation and pyroptosis via the HSP60-NF-κB axis
Article Snippet: .. After washing, the membrane was incubated with the appropriate fluorescent secondary antibody (1:7500, Abbkine, USA) at room temperature for 1 h in the dark. .. Protein bands were visualized and quantified using the Odyssey infrared imaging system (LI-COR Biosciences, USA).

Article Title: Histone demethylase JMJD1A protects mice from enteric bacterial infection by upregulating CCL8 expression to recruit macrophages and CD4+ T cells.
Article Snippet: For immunofluorescence staining (IF), the antibodies Rabbit anti-Mouse-CCL8 antibody (Cat#AF790, R&D Systems) was incubated for overnight at 4 °C. .. The Fluorescent Secondary Antibody (Cat#A23330, Abbkine) for immunofluorescence was incubated for 30 min. .. Slides were mounted with the Antifade Mounting Medium with DAPI (Cat# H-1200, Vector Laboratories, USA).

Staining:

Article Title: Interleukin-2/anti-interleukin-2 complex attenuates inflammation in a mouse COPD model by expanding CD4 + CD25 + Foxp3 + regulatory T cells.
Article Snippet: Background and purpose: Chronic, nonspecific inflammation of the alveoli and airways is an important pathological feature of chronic obstructive pulmonary disease (COPD), while sustained inflammatory reactions can cause alveolar damage.. Regulatory T cells (Tregs) inhibit inflammation, whereas the interleukin-2/antiinterleukin-2 complex (IL-2C) increases the number of Tregs; however, whether the IL-2C has a therapeutic role in COPD remains unknown.. Therefore, this study investigated whether IL-2C alleviates lung inflammation in COPD by increasing the number of Tregs.

Membrane:

Article Title: TRPM4 modulates endothelial inflammation and pyroptosis via the HSP60-NF-κB axis
Article Snippet: .. After washing, the membrane was incubated with the appropriate fluorescent secondary antibody (1:7500, Abbkine, USA) at room temperature for 1 h in the dark. .. Protein bands were visualized and quantified using the Odyssey infrared imaging system (LI-COR Biosciences, USA).



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The results of dental calculus mineralization assays. ( A ) <t>Fluorescent</t> stereomicroscopy images taken from canine dental calculi. PMA-treated neutrophils showed colocalization of ecDNA and calcified nidi, while the other groups did not. Control, untreated group; PMA, group treated with PMA (500 nM); PMA + DNase I, group treated with PMA (500 nM) and DNase I (100 U/mL). Scale bars, 1 mm. ( B ) Effect of NETs on mineralization of canine dental calculus ( n = 6). ( C ) Effect of neutrophil count on mineralization of canine dental calculus ( n = 6). ( D ) Effect of NET administration frequency on mineralization of canine dental calculus ( n = 10). ( E ) Effects of mineral ion concentration and NETs on mineralization of canine dental calculus ( n = 6). * p < 0.05, ** p < 0.01, **** p < 0.0001; ns, not significant.
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The results of dental calculus mineralization assays. ( A ) <t>Fluorescent</t> stereomicroscopy images taken from canine dental calculi. PMA-treated neutrophils showed colocalization of ecDNA and calcified nidi, while the other groups did not. Control, untreated group; PMA, group treated with PMA (500 nM); PMA + DNase I, group treated with PMA (500 nM) and DNase I (100 U/mL). Scale bars, 1 mm. ( B ) Effect of NETs on mineralization of canine dental calculus ( n = 6). ( C ) Effect of neutrophil count on mineralization of canine dental calculus ( n = 6). ( D ) Effect of NET administration frequency on mineralization of canine dental calculus ( n = 10). ( E ) Effects of mineral ion concentration and NETs on mineralization of canine dental calculus ( n = 6). * p < 0.05, ** p < 0.01, **** p < 0.0001; ns, not significant.
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Image Search Results


The results of dental calculus mineralization assays. ( A ) Fluorescent stereomicroscopy images taken from canine dental calculi. PMA-treated neutrophils showed colocalization of ecDNA and calcified nidi, while the other groups did not. Control, untreated group; PMA, group treated with PMA (500 nM); PMA + DNase I, group treated with PMA (500 nM) and DNase I (100 U/mL). Scale bars, 1 mm. ( B ) Effect of NETs on mineralization of canine dental calculus ( n = 6). ( C ) Effect of neutrophil count on mineralization of canine dental calculus ( n = 6). ( D ) Effect of NET administration frequency on mineralization of canine dental calculus ( n = 10). ( E ) Effects of mineral ion concentration and NETs on mineralization of canine dental calculus ( n = 6). * p < 0.05, ** p < 0.01, **** p < 0.0001; ns, not significant.

Journal: Veterinary Sciences

Article Title: Neutrophil Extracellular Traps Promote the Formation of Canine Dental Calculus

doi: 10.3390/vetsci13060593

Figure Lengend Snippet: The results of dental calculus mineralization assays. ( A ) Fluorescent stereomicroscopy images taken from canine dental calculi. PMA-treated neutrophils showed colocalization of ecDNA and calcified nidi, while the other groups did not. Control, untreated group; PMA, group treated with PMA (500 nM); PMA + DNase I, group treated with PMA (500 nM) and DNase I (100 U/mL). Scale bars, 1 mm. ( B ) Effect of NETs on mineralization of canine dental calculus ( n = 6). ( C ) Effect of neutrophil count on mineralization of canine dental calculus ( n = 6). ( D ) Effect of NET administration frequency on mineralization of canine dental calculus ( n = 10). ( E ) Effects of mineral ion concentration and NETs on mineralization of canine dental calculus ( n = 6). * p < 0.05, ** p < 0.01, **** p < 0.0001; ns, not significant.

Article Snippet: An anti-rabbit fluorescent secondary antibody (AS011, Abclonal, 1:100) was incubated at room temperature for 1 h in the dark.

Techniques: Control, Concentration Assay